mouse anti human cd86 pe  (R&D Systems)

Journal: PLoS ONE

Article Title: CD105 + -mesenchymal stem cells migrate into osteoarthritis joint: An animal model

doi: 10.1371/journal.pone.0188072

Figure Lengend Snippet: A) Immunofluorescence analysis for CD68 of sections from synovial membrane and crossed ligaments of the animals IV injected with DiO-CD105 + -MSCs expressing cells. All the sections are from the left knee which was injured to induce OA pathology (magnification were 10x, 20x and 40x). B) Immunofluorescence analysis for SDF-1 of sections from synovial membrane and crossed ligaments of animals IV injected with DiO-CD105 + -MSCs expressing cells. All the sections are from the left knee with OA pathology (magnification were 10x, 20x and 40x). C) Histogram showed percentage SDF-1 positives signal normalized with DAPI signal from knee sections of animals group IA injected with DiO-CD105 + -MSCs and knee sections from animals group IV injected with GFP-CD105 + -MSCs. AnalySIS Image Processing was used. * P value less than 0.05 was considered statistically significant by Mann Whitney and Kruskall-Wallis.

Article Snippet: Other sections were immunostained with antibodies: FITC mouse anti-human CD105 (1:100 from Serotec, Bavaria, GER), PE-Cy5-conjugated mouse anti-human CD90 (1:20 from BD Pharmagen, Madrid, SP), FITC mouse anti-human CD44 (1:100 from Serotec, Bavaria, GER), PE-conjugated mouse anti-human CD68 (1:50; from DakoCytomation) and PE-conjugated mouse anti-human SDF-1 (1:100; Sta.

Techniques: Immunofluorescence, Injection, Expressing, MANN-WHITNEY